The Florence-based club said that Argentine international defender German Pezzella and on-loan Wolves forward Patrick Cutrone were both tested after showing “some symptoms” of the disease along with physiotherapist Stefano Dainelli, who also tested positive.
“They are all in a good state of health in their homes in Florence,” the club said in a statement. Their teammate Dusan Vlahovic tested positive on Friday.
Five players at Sampdoria and one at Juventus have also tested positive.
Pezzella, who has won 16 caps for Argentina, is in his third season at Fiorentina and has made 94 appearances.
Cutrone was signed on loan from Premier League Wolves in January.
Fiorentina’s most recent opponents were Udinese who have suspended training and said that their squad will remain in self-isolation until March 22.
Serie A has been suspended until at least April 3 amid a general ban on sporting events in the country which has been the worst-affected in Europe by the coronavirus.